Iminothiolane hydrochloride
http://www.bjbalb.com/html/Other-Biochemical-Reagent/Y17336.html WitrynapH 9 with DIPEA, and 2 eq. of 2-iminothiolane hydrochloride per polymer were added. The mixture was stirred for 15 min at room temperature, 2 eq. of dye maleimide in PBS were added, and the solution was stirred for 5 days at room temperature. The solvent was removed and the crude product was subjected to ultrafiltration in water.
Iminothiolane hydrochloride
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WitrynaS-Nitrosothiol-modified chitosan oligosaccharides were synthesized by reaction with 2-iminothiolane hydrochloride and 3-acetamido-4,4-dimethylthietan-2-one, followed by thiol nitrosation. The resulting nitric oxide (NO)-releasing chitosan oligosaccharides stored ∼0.3μmol NO mg(-1) chitosan. Both the chemical structure of the nitrosothiol (i ... Witrynaand 2-iminothiolane hydrochloride (Traut’s reagent).29 The chitosan:Traut’s ratio determines the amount of free thiol groups (Table 1) in the thiolated chitosan (TCS) samples. TCS/ChA hydrogels were prepared by laccase-assisted oxida-tion of ChA in a one-step reaction. Controls with the same
2-Iminothiolane reacts with primary amines efficiently at pH 7 to 9, creating amidine compounds with a sulfhydryl group. Thus it allows for crosslinking or labeling of molecules such as proteins through use of disulfide or thioether conjugation. It was first used to thiolate a subunit of ribosome in E. coli in 1973 by Robert Traut, its namesake, and his colleagues. Witryna2-Iminothiolane hydrochloride Specifications Reagent grade, ≥98% (TLC), powder Synonym (Chinese) ... (2-IT.HCl)是一种蛋白质修饰试剂,通常用于将硫醇基团连接到蛋白质和肽上。 用2-IT.HCl修饰的毛发角蛋白纤维的拉曼光谱分析显示了新的二硫化物基团的形成。 性质(Properties): ...
Witryna2-Iminothiolane hydrochloride is a reagent for the introduction of sulphydryl groups into oligosaccharides derived from asparagine-linked glycans [1] . Room temperature in continental US; may vary elsewhere. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) Witryna2-Iminothiolane hydrochloride Revision Date 23-Dec-2024 4.1. Description of first aid measures General Advice If symptoms persist, call a physician. Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Get medical attention. Skin Contact Wash off immediately with plenty of water for at least …
WitrynaAlternate Names: Traut's Reagent. Application: 2-Iminothiolane, Hydrochloride is a thiolane derivitization agent. CAS Number: 4781-83-3. Purity: ≥98%. Molecular …
WitrynaCAS: 4781-83-3 MDL: MFCD00039013 Synonyms: 2-IT 2-Iminiothiolane hydrochloride is a thiolating reagent that is used in the preparation of disulfide and thioether linked … fisher price frozen playsetWitryna请注意,蛋白质(例如抗体)可能因二硫键的完全还原而失活。选择性还原IgG铰链区中二硫键的可以用2-巯基乙胺·HCl完成。可以使用SATA(N-succinimidyl S-acetylthioacetate)或2-iminothiolane·HCl(Traut's Reagent)将巯基加成到蛋白分子中,修饰伯胺。 使用方 … can alliums grow in shadeWitrynaThe activity of the antibodies immobilized on the microcantilever via protein A was 1.7-fold of that via the sulfhydrylation reagent 2-iminothiolane hydrochloride. A microcantilever immunosensor method with protein A as the functionalization reagent was established to detect the residues of clenbuterol and chloramphenicol at limits of … can all living things get cancerWitryna15 mar 2024 · To determine the surface accessible primary amino moiety on N RE-MPC, the facile colorimetric method using 2-iminothiolane hydrochloride (ITL) as a molecular probe coupling with bicinchoninic acid (BCA) working solution was carried out as described in SI (Scheme S1 and Fig. S1). This method is based on the reaction of … fisher price fruit marketWitryna2-Iminothiolane 2-Iminothiolane Hydrochloride is commercially available in GMP quality and is commonly used in the PEGylation of therapeutic proteins. Uses. For the transformation of a primary amine to a sulfhydryl group in drugs and therapeutic proteins. For conjugation with monoclonal antibodies, proteins, peptides and herbicides. can all iphones use the same chargerhttp://www.haoranbio.com/ProductDetails-653340.aspx can allium be planted in springWitrynaSAFETY DATA SHEET Creation Date 13-Dec-2012 Revision Date 28-Dec-2024 Revision Number 4 1. Identification Product Name 2-Iminothiolane hydrochloride Cat No. : AC450180000, AC450180010, AC450182500 CAS No 4781-83-3 Synonyms 2-Thiolanimine hydrochloride; Traut's reagent Recommended Use Laboratory … fisher price frog seat