WebThe promoterlessgfpgene was isolated as a 820-bpPst I fragment from pFPV27 (7) and inserted in the same orientation into the PstI site upstream of cat. Following digestion by … WebPlasmid pBS532 promoterless GFPcre from Dr. Brian Sauer's lab contains the insert GFP-cre and is published in Anal Biochem. 1999 Jun 1. 270(2):334-6. This plasmid is available …

Promoterless Reporter Vectors OriGene

WebJun 3, 2024 · We constructed a polycistronic reporter vector containing the ORFs of four well-known reporter genes ( Rluc, Fluc, GFP, and RFP ). An RNA activator targeting region … WebVPK-411 pAAV-MCS Promoterless 4 VPK-415 pAAV-IRES-Puro 1.8 VPK-416 pAAV-IRES-Neo 1.6 VPK-417 pAAV-IRES-Hygro 1.4 VPK-418 pAAV-IRES-GFP 1.7 VPK-419 pAAV-IRES-Bsd 2 Table 1. Packaging capacity of AAV shuttle vectors. Recombinant adeno-associated viruses are important tools for gene delivery and expression. AAV has cena metala na otpadu

What Is a Promoter? How It Works and Criticism of Promoters

WebMay 12, 2013 · We introduced TALEN pair 2, along with a donor construct that contained a promoterless GFP and a puromycin selection marker flanked by short homologous arms (700 bp and 385 bp for 5′ and 3′ arms,... WebAug 16, 2005 · Now Shaked et al.() report that the Saccharomyces cerevisiae Rad54 protein substantially boosts GT frequencyThese authors devised an ingenious GT assay by using a promoterless GFP gene inserted in an Arabidopsis cruciferin gene. Cruciferin is a seed storage protein; hence, integration of the GFP-containing gene into the one of the genomic … WebApr 5, 2007 · BamHI cuts at a single site at the 5′ end of the promoterless gfp gene, allowing for the screening of DNA fragments for promoter activity. Resolve the restriction digested DNA on a 0.8% ... cena mesa za sušenje