Thp1 isre

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August undefined, 2024

WebThe CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells.To construct this cell line, the SIE-bla construct was transduced into THP-1 cells by lentivirus.Flow cytometry was used to isolate cells responsive to Interferon Gamma (IFNγ). This cell line … Webof five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-κB or AP-1 pathways. As a result, THP1-Lucia™ …

THP1-Lucia NF-κB Cells NF-κB Reporter monocyte InvivoGen

WebDec 4, 2024 · Mária Csumita, Attila Csermely, Attila Horvath, Gergely Nagy, Fanny Monori, Loránd Göczi, Hans-Acha Orbea, Walter Reith, Lajos Széles, Specific enhancer selection by IRF3, IRF5 and IRF9 is determined by ISRE half-sites, 5′ and 3′ flanking bases, collaborating transcription factors and the chromatin environment in a combinatorial fashion, Nucleic … WebThe CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells.To construct … glyn garth anglesey

Regulation of monocyte induced cell migration by the RNA binding ...

WebSep 9, 2015 · IFN in THP1 supernatants was assessed by bioassay. Gray wedges represent a 1:2 dilution series starting with extract from 10 7 infectious units. As controls, synthetic 2′3′-cGAMP was either directly added to THP1 cells (grey bars) or was spiked into medium and then included in the extraction procedure (orange bars). WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase … WebDec 1, 2024 · In contrast, in THP1, ISRE bound by STAT1 or STAT2 accounted for 11 % and 77 %, respectively, of bound ISRE, possibly reflecting sensitivity issues in ChIP antibody … bollow stadt winsen

Interferon stimulated binding of ISRE is cell type specific …

IRF Reporter (Luc) – THP-1 Cell line (cGAS-Sting Signaling Pathway)

WebThe stimulator of interferon genes (STING) is a critical protein in the activation of the immune system in response to DNA. It can participate the inflammatory response process by modulating the inflammation-preferred translation program through the STING-PKR-like endoplasmic reticulum kinase (PERK)-eIF2α pathway or by inducing the secretion of … WebFor the interferon-stimulated response element (ISRE)-luciferase assay, HEK293 cells were seeded (1 × 10 5 ml-1) onto 96-well plates 24 h prior to transfection, and 80 ng of ISRE … bollow hill farm fromeWebOct 2, 2024 · Using a recently developed macrophage cell system for large-scale perturbation screening (Sun et al., 2016, Li et al., 2015) (Figure 1 A), we conducted a … bollox discount code

"WebAll the THP1 cells (parent, MAVS, and STING KO cells) were seeded each at cells per well in a 100 μL complete growth medium containing 100 nM PMA (phorbol 12-myristate 13-acetate) in 96-well plates. At 72-hour postdifferentiation, the derived macrophages were treated with 0.5–4 μ M cryptolepine in a 100 μ L PMA-free complete growth medium. " - Thp1 isre

Thp1 isre

WebTHP-1 ISRE-Luc2 IFN Stimulation Concentration (ng/mL) Fold Change 1 10 100 1000 0 10 20 30 40 50 THP-1 ISRE-Luc2 IFN Stimulation Concentration (ng/mL) Fold Change 0.1 1 10 … WebDescription. The Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in the human monocytic cell line THP-1. It contains a firefly …

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WebTHP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. THP1-Lucia™ cells feature the Lucia gene, a … WebJul 17, 2024 · Instead, HeLa and K562 shared roughly 60 % 60 % of their bound ISRE while THP1 shared 20 % 20 % of bound ISRE with each of the other cell types. As in mouse cell …

WebJun 6, 2024 · These results suggested that tryptanthrin specifically regulates the TLR3/IRF3/IFN-β axis in THP-1 Mϕ. Fig. 4. Cells were stimulated with 20 μg/mL poly IC ( A) or 1 μg/mL LPS ( B) with 0.01–1 μM tryptanthrin for 2 h or 1 h, respectively. The cell lysates were subjected to western blotting for detecting p-IRF3 and IRF3. WebJan 6, 2016 · A maximum of TAP1 induction in THP1 cells was seen after stimulation with 200 U/ml IFNα. Initial time course experiments indicated that the stimulatory effect of IFNα increases up to 6 h of treatment, then declines. However, enhanced levels of TAP1 expression could be observed for at least 48 h in THP1 and A375 cells (data not shown).

WebSep 16, 2024 · THP1 cells were left uninfected or infected with HSV-1 for the indicated times before endogenous coimmunoprecipitation and immunoblot analysis with the indicated antibodies. ... Reporter assays indicated that mutation of Y167, Y240, or Y245 of MITA abolished its ability to activate ISRE (SI Appendix, Fig. S6B). Previously, ...

WebHTRF Quantification of IFN-b, IL1-b, and TNF-a in THP1 cells THP1 WT-cells were seeded at 1,000,000 cells per well in 275µL RPMI supplemented with 10% FCS. After incubation at 37°C, 5% CO2, the cells were stimulated or not with 30µL of the STING agonist 2’3’ cGAMP used at 400µM final. Stimulation was performed for 24 hours, then either ...

WebISRE promoter. Luciferase Expression ATCC® Number TIB-202-ISRE-LUC2™ Organism Homo sapiens Tissue/Disease Source Acute Monocytic Leukemia Product Description … glyn garth chesterWebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … glyn garth court flatsWebIRF5 knockout dual reporter monocytes. THP1-Dual™ KO-IRF5 cells were generated from the THP1-Dual™ cell line through the stable knockout of the IRF5 gene.They feature two … bollox discount codesWeb1 ISRE-Luc/NFκB-SEAP/THP-1 CBP74097 I.Background THP1reportercellsarederivedfromTHP-1,ahumanmonocyticcellline thatnaturallyexpressesmanypattern-recognitionreceptors ... glyn fryWebTHP1-Blue® ISG cells were specifically designed for monitoring the interferon signaling pathway in a physiologically relevant cell line. THP1-Blue® were derived from the human … bollow und hinrichsWebthe migration of another THP1 monocyte cell line that stably expresses GFP for visualization.41 The top chambers of the transwells were ﬁlled with equal numbers of a THP1 cell line Figure 2. Global gene expression proﬁling of FXR1 regulated mRNAs by microarray analysis in control and FXR1 knockdown THP1 monocytes. (A) (Left) Heatmap … glyn garth guest house aberystwythWebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter … glyn garth court for sale